Competitive binding of aristolochic acid between various cyclodextrins and serum albumin as a model for acute poisoning detoxification

We showed that aristolochic acid toxin (ARI) and its fluorescent analogue form inclusion complexes with various cyclodextrins (CDs), including the clinically used γ-CD: Sugammadex. The binding affinity of ARI towards CDs varied on a CD size rim modifications, whereby stability ARI/Sugammadex complex was 2-fold greater than to serum albumin (BSA), blood protein well-known as transporter small molecules similar ds-DNA. Molecular modelling supported formation ARI/ Sugammadex carboxyl group exposed water ARI-condensed aromatic moiety deeply immersed into CD-cavity. These results are proof-of-concept could be first aid upon acute intoxication by larger part in competition or ds-DNA significantly reducing absorption organism, similarly extracts drugs from stop their bioactivity.